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KMID : 0043320120350061015
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Archives of Pharmacal Research
2012 Volume.35 No. 6 p.1015 ~ p.1019
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A convenient method for the enantiomeric separation of α-amino acid esters as benzophenone imine Schiff base derivatives
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Huang Hu
Xu Wen Jun
Jin Jing Yu
Hong Joon-Hee
Shin Hyun-Jae
Lee Won-Jae
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Abstract
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A convenient liquid chromatographic method for the separation of α-amino acid esters as benzophenone Schiff base derivatives on coated chiral stationary phases (CSPs) (Chiralcel OD-H, Chiralcel OD, Chiralpak AD-H, Chiralpak AD, and Chiralpak AS) or covalently immobilized CSPs (Chiralpak IA, Chiralpak IB, and Chiralpak IC) derived from polysaccharide derivatives is described. Benzophenone imine derivatives of α-amino acid esters were readily prepared by stirring benzophenone imine and the hydrochloride salts of α-amino acid esters in 2-propanol. The chromatographic separations were conducted at a flow rate 1.0 mL/min and a detection wavelength of 254 nm; 0.5% 2-propanol/hexane (v/v) was used on CSPs. In general, the resolution of Chiralpak IC was superior to those of the other CSPs. In addition, the resolutions of other arylimine derivatives of α-amino acid esters and the effects of different mobile phases on the enantiomeric separation of α-amino acid esters as benzophenone imine derivatives on Chiralpak IC were investigated.
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KEYWORD
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Chiral stationary phase, Benzophenone imine derivative, Enantiomer separation
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